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Check solvent pH and adjust with buffer (e.g., PBS) if required

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Riezzo I, Cerretani D, Fiore C, Bello S, Centini F, DErrico S, Fiaschi AI, Giorgi G, Neri M, Pomara C, Turillazzi E, Fineschi V (2010) Enzymaticnonenzymatic cellular antioxidant defense systems response and immunohistochemical detection of MDMA, VMAT2, HSP70, and apoptosis as biomarkers for MDMA (ecstasy) neurotoxicity

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