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All analyses were performed by a researcher blinded for genotype and age of the mice, and all recordings and images were analyzed in a random manner

Effects of metformin on the gut microbiota: a systematic review

Lancet (2015) 386:98394

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For a 14-day single-subject study with daily 100g injections, a 2mg DSIP vial provides 20 doses when reconstituted at 1mg/mL concentration sufficient for the 14-day protocol with six extra doses as a buffer for measurement error or protocol extension

10.1089/zeb.2009.0639 10 CoffinA