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Be careful not to touch the needle to keep it clean
For immunofluorescence staining, brain tissues were dissected out, post-fixed by 4% paraformaldehyde (PFA) for 12 h, embedded in paraffin, cut into 5 mm sections and mounted on slides for subsequent staining
Antioxidants Redox Signal (2020) 33(2):5965
Future studies should therefore aim for reproduction of these findings in larger samples, preferentially in multi-center studies
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In particular, leptin modulates the signals for satiety found in the ARC
