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Then, non-irradiated and irradiated samples were centrifuged and the pellet was fixed with 2% glutaraldehyde and 1% paraformaldehyde in 0.1 M cacodylate buffer for 1 h at room temperature and then overnight at 4C

Unlike muscles or skin, discs have very limited blood supply

In a small RCT in China (n=39) with patients hospitalised with community-acquired pneumonia, the addition of high dose NAC (1200mg/d for 10 days) to usual care suggested an improvement in oxidative stress parameters, inflammatory factors but not radiological changes compared to usual care only

Meena D, Jha S

In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm