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NPC differentiation to dopaminergic neurons NSCs were grown at high confluence (70%) for 7 days on Matrigel-coated dishes in N2B27 medium supplemented with 200 ng/ml Sonic Hedgehog and 100 ng/ml FGF8 (100-25

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Funding This work was supported by the Anhui Provincial Department of Science and Technology [Grant number 202007d07050007 and 2208085MH214