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The membrane was incubated with rabbit anti-GPX1 antigen affinity purified polyclonal antibody (Catalog # PB9203) at 0.5 g/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT

31,32 Regarding the importance of these polymorphic genes, we conducted this study to understand whether the polymorphisms of these three genes or their combinations have any effect on disease progression or susceptibility

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Gradual dose increases or titration is used to reduce side effects and improve the tolerabily of the medication

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