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Inflammation-induced neurotransmitter dysregulation Chronic inflammation significantly disrupts neurotransmitter balance in EDC, not only altering homeostasis but also affecting major neuromodulatory systems, including 5-HT, dopamine (DA), GABA, and glutamate (GLU)

J Histochem Cytochem 27:756762 Patel AJ, Weir MD, Hunt A, Tahourdin CS, Thomas DG (1985) Distribution of glutamine synthetase and glial fibrillary acidic protein and correlation of glutamine synthetase with glutamate decarboxylase in different regions of the rat central nervous system

3) Add 1ml Binding/Wash Buffer to the tube and mix well, use the magnetic separation rack to collect the beads and discard the supernatant

Cells were then exposed to BSO and different inhibitors for 14 h, and then rinsed three times with Hank's balanced salt solution (HBSS)

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G6PD phosphorylation 60 , acetylation 61 and O-GlcNAcylation 62 have been reported previously