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Theranostics 11 , 77797796 (2021)

The extensive degeneration of these DA neurons results in diminished dopamine levels within the brain, which gives rise to a spectrum of clinical manifestations, encompassing challenges in maintaining posture, the emergence of stationary tremors, a decrease in movement speed (bradykinesia) and the onset of joint stiffness reminiscent of ankylosing arthritis (49)

Briefly, PE and PTC snap-frozen tissue was pulverized in liquid nitrogen and homogenized in RIPA buffer (150 mM NaCl, 50 mM Tris, 1% NP-40, pH 7.5)

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Rights and permissions About this article Cite this article Schulz, E., Gori, T

Ostrom QT, Cioffi G, Waite K, Kruchko C, Barnholtz-Sloan JS