glutathione resin binding Xpure Agarose - Protein Expression / Purification Reagents columns and binding/wash and elution buffers are available separately Featured PurKine™ GST-Tag Protein Purification

Glyoxal Low Density Agarose Resin (4%) Cross-linked Glyoxal Low Density Agarose Resin (4%) Cross-linked consist of 4 percent agarose beads with size of 50-150 m that allow a covalent binding of agarose to lysine amino groups

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Samples were measured in duplicate, blank values were subtracted, and a standard curve was obtained for each plate using Microsoft excel, optical densities were converted to concentrations in pg/mL

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doi:10.1038/s41586-020-1935-3

Wild type OLFM4, or OLFM4 deleted of the signal peptide (DelSP) (1-20 aa) were cloned into pCDNA3.1(+) vector