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A continued, unresolved imbalance between effector and regulatory immune responses further exacerbates inflammation that ultimately causes tissue and organ damage

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In glycolysis, PYR is the final cytosolic product of glucose metabolism and serves as a key intermediate, undergoing oxidative decarboxylation by the PDH complex to generate acetyl-CoA

Expected Results Timeline Understanding realistic timelines prevents frustration and premature protocol abandonment

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Cell proliferation was evaluated using a Promega CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) as per the manufacturers instructions (Promega G3580)