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doi: 10.1016/j.freeradbiomed.2005.03.032

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Preparation of conditioned medium The medium was refreshed upon the cells met 50% confluence, and the conditioned medium (CM) was obtained by filtering the culture medium through 0.45 m microporous membrane after 48 h of incubation 8

Overexpression of PSAT1 regulated by G9A sustains cell proliferation in colorectal cancer