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10.1016/j.earlhumdev.2022.105588 25 BraunschweigD.Van De WaterJ

Methionine oxidation in the calmodulin-binding domain of calcineurin disrupts calmodulin binding and calcineurin activation

First, only 144 of the required 790 couples were recruited to achieve 80% power for the primary outcome (live-birth rates)

Formation of hydrogen peroxide was determined after addition to the solution of 10 l of 2-7dichlorofluorescein diacetate (2m in ethanol) and DCF fluorescence measured as described previously (Garca-Ruiz et al, 1995, 1997)

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After reconstitution: Store at 28 C , protect from light, and avoid repeated temperature cycling
