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The rapid cysteine clearance during fermentative growth is consistent with previous reports showing that the thiol group of supplemented cysteine is converted into H 2 S/sulfane sulfur in fermenting yeast (Winter et al., 2014), a process mediated by enzymes such as 3-mercaptopyruvate sulfurtransferase (TUM1 in yeast, (Huang et al., 2016)) and potentially by enzymes of the transulfuration pathway (Filipovic et al., 2018) or cysteinyl-tRNA synthetase CRS1(Nishimura et al., 2024), although direct evidence for H 2 S/sulfane sulfur production upon cysteine addition by the transulfuration/CRS1 enzymes in yeast has not been yet demonstrated

doi: 10.4103/0975-9476.146563

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GSSG: Oxidized Glutathione