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For each condition 1 mL supernatant was collected from the 150 cm 2 flask, centrifuged at 10,000 g and 4 C for 1 min, 500 L were transferred into a 1.5 mL collection tube, snap-frozen in liquid nitrogen, and stored at 80 C until analysis

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cerevisiae ), is higher compared to that of the in vitro model

Figure 1 The impact of stress on drug metabolism is unique and distinct from that of medicines that usually display dose- and time-dependent specificities ( CYP2E1 and CYP2B constitutive expression, whereas it up-regulated most of the other CYPs including those belonging to CYP1A, CYP2A, CYP2C, CYP2D and CYP3A subfamilies and metabolize over 70% of prescribed drugs (69)

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