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The supernatant was transferred to a 96 well plate and melanin absorbance at 4 l 0nm was measured using a plate reader
At 1 g/ml, hTERT expression was upregulated 12-fold in 21NT and at 0.5 g/ml, BT474 showed a fivefold upregulation relative to the untreated cells (Fig
doi: 10.1016/j.jad.2007.04.024
MennigenRNolteKRijckenEUtechMLoefflerBSenningerNet al
After cultivation for 24 h, cell viability was investigated with CellTiter 96 Aqueous One Solution (Promega Corporation)
Purcell, M., et al
