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For protein purification, harvested cells were resuspended with ice-cold lysis buffer (50 mM Tris-Cl pH 8.0, 300 mM NaCl, and cOmplete, EDTA-free protease inhibitor cocktail tablets (Roche)) at a ratio of 3.2 mL/g of cell pellet

More robust, heterogeneous PDMCTs available to screen novel therapeutics during preclinical development could greatly improve the quality of novel therapies entering clinical trials

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A systematic search was conducted across PubMed, Scopus, and Web of Science databases, utilizing a predefined set of keywords related to malnutrition, epilepsy, and nutritional interventions

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