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Protein lysates were made up at 0.75 g/L in sample buffer (50 mM Tris-HCl pH 6.8, 2% SDS, 0.1% bromophenol blue, 10% glycerol) with the addition of 5% -mercaptoethanol

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Luchetti and collaborators [135] carried out a multicentric observational study on 377 patients suffering from CTS to evaluate the management practices of this pathology in Italy

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