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For longer-term laboratory storage, freezing at approximately 20C is commonly referenced in research settings to help maintain compound integrity

For example, the Vegetarian Nutrition Dietetic Practice Group recommends that vegans consider supplementing with 250 mcg of B12 per day

This can benefit individuals with severe deficiencies or those needing a quick boost in their B12 levels

Patients with severe megaloblastic anemia occasionally develop hypokalemia (low potassium) when beginning treatment

Common Side Effects When side effects occurred, they were typically mild, transient, and similar in frequency to placebo groups: Reported Adverse Effects Most Common (occurring in small percentage of participants): Mild to moderate headaches (most frequently reported, often resolving with continued use) Injection site reactions (minor redness, swelling, or irritation at injection site) Gastrointestinal effects (particularly at higher oral doses: mild nausea, diarrhea, flatulence) Fatigue (reported occasionally during initial adaptation period) Rare Events: One case of chest tightness was deemed possibly related to treatment in safety trials

Adequate protein (0.8-1g per pound of body weight) preserves lean mass while the deficit targets fat