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Nonetheless, an isotope tracer analysis found that only serine, not glycine, feeds 1C metabolism in cancer cells in vitro [48]

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[DOI] [PubMed] [Google Scholar] 149.De Mattia G., Bravi M.C., Laurenti O., Cassone-Faldetta M., Proietti A., De Luca O., Armiento A., Ferri C

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Then, lysis buffer (1% NP-40, 10% Glycerol, 137 mM NaCl, 20 mM Tris pH7.6, 20 mM NaF, 2 g/mL aprotinin, 2 g/mL leupeptin, 2 g/mL pepstatin, 200 M Na3VO4, 1 mM PMSF) was added and cells were stored at 4 C for 30 min

Arthritis Rheum: Official Journal of the American College of Rheumatology (2006) 54:152935