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200,000 cells were plated into a 6 well dish for each knockout condition and respective controls
2021;54:e13106
2011 Jun;86(6):484-9
We also found no involvement of FGF-2 in Lu99 cell proliferation in vitro, but further studies are needed to confirm whether FGF-2 inhibition by its neutralising antibodies and multi-tyrosine kinase inhibitors targeting FGF receptors suppresses tumour growth of NSCLC cells including A549 cells, in which serum-free culture could not be adapted and FGF-2 expression was extremely lower than in Lu99 cells, through in vivo assays to test the anti-angiogenic effect
However, these tools are still in the investigational stage, and their transition into clinical practice demands rigorous validation through large-scale, multicenter studies
Methods 109 , 311 (2016)
