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The activation of PLC results in the release of inositol-1,4,5-trisphosphate (IP 3 ) from membrane associated phosphatidylinositol-4,5-bisphosphate (PIP 2 ), which then binds to specific IP 3 receptors in the endoplasmic reticulum/sarcoplasmic reticulum (ER/SR) resulting in the release of intracellular stores of Ca 2+

Tricyclic antidepressants, such as cyclobenzaprine are dosed at 520 mg or nortriptyline, initiated at a dose of 10 mg at bedtime, while SNRIs like duloxetine and venlafaxine are started at 30 mg and 37.5 mg daily, respectively, titrated to a maximum of 50% of that used in non-cirrhotic population

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