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Most of the efflux was suppressed by inhibition of xCT, and a smaller fraction by GPNA in U87-MG (Fig

Marx, N

Gullberg, R.G

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Finally, the fractionated polysaccharides were purified through the Sephadex G-100 column (2.5 60 cm) and then concentrated, dialyzed, and lyophilized

After incubation at 37 C with shaking (500 rpm) for 1 h., the cells were pelleted at 5,000 x g for 10 min at 4 C, washed once with ice-cold phosphate-buffered saline (PBS, pH 7.4), resuspended in 200 L of cold TE lysis buffer (30 mM TrisHCl, 1 mM EDTA, pH 8.0), and transferred to Lysing matrix B tubes (MP Biomedical)