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Briefly, eicosanoids were purified using strata-x polymeric reverse-phase columns (8B-S100-UBJ Phenomenex), reconstituted in buffer A, and analysed by reversed-phase ultraperformance liquid chromatography with mass spectrometry using a Sciex 6500 Qtrap mass spectrometer using previously described methods 72
Application of this technology in the transparent zebrafish embryos with the fluorescent protein reporter genes enables unparalleled visual analysis of gene expression in a living organism
Oct 2020;31(10):2017-2024
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10.3390/biom11050700 78 RosenkransZ
Subsequent redox cycling of the NFT radical generates superoxide (O 2 ) and O 2 -derived reactive oxygen species (Wardman 1985
