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Step 3: Support the recycling process

The genetically-encoded biosensor proved to be versatile, as exemplified by its functionality in a series of evolutionary distant organisms (bacteria, plants and animals), different subcellular localizations (cytosol, mitochondria, chloroplasts) and in combination with nutritional, metabolic, pharmacological and genetic perturbations

The tube was identified as a major obstacle as the lipoplexes adsorbed to the tubing, resulting in lossoffunction of the mRNAlipoplexes

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