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Using alcohol swabs on vial tops, never touching needle tips, and avoiding multi-use of single-use components maintains safety

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Statistical analysis Analysis of Variance (ANOVA) was used to analyze the data and Duncan Multiple Range Test was performed where significant difference was observed in the mean heavy metal concentrations of parasite, liver and intestine while T-test was used to compare mean concentrations of heavy metals between water and sediment, and between activities of enzymes between parasitized and non-parasitized fishes using SPSS 20 software