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The aatJ - lacZ and aatQ - lacZ fusions were then excised from pJMS07 and pJMS08 through double digestion with Xba I- Spe I, and the liberated fragments were separately cloned into the Xba I site of the promoter-less plasmid P lac -pBBR1MCS-5 [13] to give pJMS09 and pJMS10, respectively

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PMID: 35022962