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Redox and pH gradients drive amino acid synthesis in iron oxyhydroxide mineral systems
Whats the damage

2.4 Antioxidant Enzymes Extraction and Assay Fresh leaf samples (0.5 g) were ground with 5 mL of ice-cold 100 mM phosphate buffer (pH 7.4) containing 1% polyvinyl pyrrolidine and 1 mM EDTA, then centrifuged at 15,000 g for 10 min at 25 C

2PAM is known to work synergistically with atropine (some 30x greater)

Furthermore, by integrating with phosphopeptide enrichment, the nanoFAC 2D RPLC platform identified 20,000 phosphopeptides from 100 g of MCF-7 cell lysate

PMID: 16930377
