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3.1.6 Variable loop engineering Utilizing structural distinctions among archaeal AARSs, mutually orthogonal PylRS/tRNA Pyl pairs were identified ( Methanosarcina barkeri (Mb) PylRS, which is absent in Methanomethylophilus alvus (Ma) PylRS, is known to bind to the T arm and the variable loop of tRNA ( Pyl could produce an orthogonal tRNA Pyl for Mb PylRS/tRNA Pyl

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Satellite growth assessments were carried out in 5% sheep blood agar (Qingdao Hope Bio-Technology Co., Ltd., China)

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