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Briefly, transformed BL21 (DE3) Codon Plus RIPL cells (Stratagene, Santa Clara, CA) were grown at 37 C and protein expression was induced at 18 C for at least two hours with 0.3 mM IPTG

Ageing Res Rev 7, 43

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GROMACS 4.5: a high-throughput and highly parallel open source molecular simulation toolkit

HO-1 in ischemic conditions The heart is enriched in the number of mitochondria due to its high metabolic demand, making it particularly sensitive to oxidative stress (158)
