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Description
Cell debris was removed by centrifugation at 30,000 g and 4 C for 45 min and the resulting crude extract was applied onto a Strep-TactinXT 4Flow column (IBA Lifesciences, Gttingen, Germany) and purified following the manufacturers instructions

PMID: 118 BabuADevi RajeswariVGaneshVDasSDhanasekaranSUsha RaniGet al

The ferrous iron content was calculated using the standard formula

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1 What is Generalized Anxiety Disorder
Research has explored its potential in the treatment of inflammatory diseases, neurodegenerative disorders, and gastrointestinal disorders (Delgado et al., 2013)
