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In vitro acetylation assay For the in vitro acetylation assay, 2 g of purified His-SUMO-ACAT1 enzyme was incubated with 2 g of substrate (either GST-DLD WT or K430R) in a 50 L reaction

Cameron AM, Castoldi A, Sanin DE, Flachsmann LJ, Field CS, Puleston DJ, et al

Modulating effects of Astragalus polysaccharide on immune disorders via gut microbiota and the TLR4/NF-B pathway in rats with syndrome of dampness stagnancy due to spleen deficiency

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This enzyme metabolizes tryptophan into the immunosuppressive metabolite kynurenine, which inhibits the therapeutically desired infiltration of tumors with cytotoxic CD8 + T cells [20]