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4.2 Iron depletion methods Free iron can catalyze the production of ROS in Fenton reaction, and excessive production of ROS in the presence of iron overload can lead to oxidative stress and damage DNA, lipids, and proteins ( DFO is a clinically used iron chelator derived from streptomyces polymyxa and is a hexadentate complex that forms a 1:1 Fe 3+ /DFO complex at physiological PH

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Selenium is also involved in the insulin signal transduction pathway affecting the expression of important metabolic enzymes

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