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Williams J, Mai CT, Mulinare J, et al

Adherent platelets were observed under an inverted fluorescence microscope (AXIO ScopeA1, Zeiss, Germany) and processed using Image J software

Since then, additional studies have confirmed the finding by showing that human primary BMAds and bone marrow stromal cell (BMSC)-derived BMAds both express and secrete leptin (273, 418, 512)

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The quality of the selected RCTs was assessed by same authors independently using the Cochrane Collaboration risk of bias tool based on the following criteria: "allocation concealment, randomization generation, outcome assessors and blinding of participants, selective outcome reporting, incomplete outcome data, and other sources of bias"