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J Androl (2012) 33(4):58893
Animals and Surgery Male (n=33) and female (n=19) Long-Evans rats were single-housed with enrichment on a 12/12-hour reverse light cycle and maintained at 85100% free-feeding weight (5% bodyweight of chow/day)

Cytosol fractions isolated through refrigerated centrifugation were incubated with a ruthenium complex (100 mg/mL of 0.02 mM DMSO solution) for 24 h at 4 C

In the GH signaling, GH binding proteins are produced at the cell surface and play complex roles, including modulate the half-life of plasma GH and the binding of GH to GHR (Cawley et al., 2013)
