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However, to date, oligonucleotide therapeutics are still facing some technological obstacles, such as how to achieve efficient oligonucleotide delivery to target organs or tissues, how to overcome off-target interactions [4952], and how to address the sequence- and chemistry-dependent toxicity and saturation of endogenous RNA processing pathways [53, 54]

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Figure 2 Previous work has demonstrated that aminoglycosides activate acute and slow (chronic) mechanisms that are distinct from one another, with neomycin only activating acute mechanisms but gentamicin activating both pathways (Coffin et al., 2009)

Mass spectrometry verifies molecular weight matches the expected compound

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Most clinics add vitamin B12