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Maternal exposure to a mixture of phthalates affected folliculogenesis and steroidogenesis in F1 and F2 generation female rats and altered the expression of miRNAs in the F1 generation ovaries of female rats (Gonsioroski et al., 2022)

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Targeting MTHFD2 to exploit cancer-specific metabolism and the DNA damage response

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Key advantages include: (i) Strong T7 Promoter: Increases protein production, (ii) Common Restriction Sites: Especially upstream of the T7 promoter, making it ideal for expression cloning, (iii) Lac Operator Regulation: Suppresses uninduced expression, (iv) Self Lac Repressor Encoding: Reduces promoter leakiness, especially beneficial for bacteria with lac repressor deficiencies and (v) Medium Copy Number (2025 per cell): allows high expression levels without overloading the cell