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2.4 RNA extraction, cDNA library construction, and transcriptome sequencing Total RNA was isolated using the mirVanaTM miRNA isolation kit, following to the manufacturer s instructions

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G-6-PD produces NADPH, which maintains the Glutathione and other proteins when the RBCs are exposed to oxidants

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Besides, icariin primarily used for male reproduction, has shown potential therapeutic and preventive effects in the nervous system and can be used as an anti-inflammatory agent for depression, etc

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