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Measurement of intracellular calcium potential For detection of intracellular calcium potential, the kidney organoids were washed with PBS and treated with a Fluo-8 calcium assay kit (5 M, AAT Bioquest) for 1 h at 37 C in darkness

They also play a role in other contexts, such as scarring, abnormal tissue growth and cancer biology

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Reconstitution Math in Published Literature Because Epithalon arrives as a lyophilized (freeze-dried) powder, researchers had to reconstitute it with bacteriostatic water before measuring it for studies

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