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Activates ERK1/2 and AKT pathways via growth factor receptor upregulation

All other reagents were purchased from Sigma-Aldrich, unless otherwise noted
For protoplast transfection, we mixed 150 L protoplasts (1.5 10 5 cells), 40 g plasmid, and 120 L buffer in a 2-mm electroporation cuvette (BioRad #1652086)

The combination treatment was more effective and faster acting than standalone fumaric acid esters

Start low, assess response over two weeks, and adjust based on subjective and objective cognitive metrics
