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[DOI: 10.1177/0379572115585772] [DOI] [PubMed] [Google Scholar] Castelli 2011 Castelli MC, Friedman K, Sherry J, Brazzillo K, Genoble L, Bhargava P, et al

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to an OD 600 of 0.7-0.8 (2-3 h) before induction with 0.5 mM IPTG and incubated for an additional 3 h