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To isolate oligotrophic bacteria, previous studies have shown that reducing nutrient concentrations in the media and extending the incubation time can be effective [22, 23]

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Many types of cells, such as endothelial cells, keratinocytes, osteoblasts, rhabdomyosarcoma cells, epithelial cells, trophoblasts, melanoma cells, breast cancer cells, smooth muscle cells, carcinoma cells and neuroblastoma cells, migrate toward a source of IGFs or display increased motility in the presence of these factors [26,27]