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The top layer was aspirated without perturbing the disk, additional MeOH (400 l) was added, and the proteins were pelleted (10,000 g, 10 min, 4C) and the pellet were resuspended in 300 L of 50 mM TEAB, containing 2 M urea by water bath sonication

doi: 10.1172/JCI8342
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Of the resultant solution, 20 L was added to a glass vial containing 300 L of 0.1 M NaOH

de Carvalho, L

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