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Matias AC, Matos J, Dohmen RJ, Ramos PC (2023) Hsp70 and Hsp110 chaperones promote early steps of proteasome assembly

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These complexes work sequentially to transfer electrons, establish a proton gradient, and drive ATP synthesis

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After complete dissolution of the gel, the sample was added to a Qiagen spin column which was then centrifuged at 9200 g for 1 min

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