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Afterward, samples were dehydrated through a graded alcohol series then paraffin-embedded, sectioned (5 m thickness), and stained with hematoxylin and eosin (H&E) to illustrate the general histological components with subsequent examination conducted under a light microscope (BX51, Olympus, Tokyo, Japan), and photomicrographs were acquired at 10X and 40X objectives (Paul and Chanda 2017)

21 These inclusions are the major constituent of intracellular protein inclusions forming the Lewy bodies and Lewy neuritis in dopaminergic neurons of the substantia nigra

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Let both the peptide vial and bacteriostatic water vial reach room temperature

Partially used vials must not be stored for later any leftover water should be thrown away, as it will not remain sterile