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(2) V3-V4 Amplification Target regions were amplified with barcoded primers 341 F (5-CCTAYGGGRBGCASCAG-3) and 806 R (5-GGACTACNNGGGTATCTAAT-3) using Phusion High-Fidelity PCR Master Mix (New England Biolabs, USA)

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These wide-ranging physiological effects occur by interaction with four G-protein-coupled receptors with seven transmembrane domains (H1, H2, H3 and H4), which activate signal transduction pathways upon perceiving their ligand, histamine [7,12]

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