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All experiments were repeated at least in triplicate

Actin staining was then performed with pre-conjugated Phalloidin-Alexa Fluor 488 (Cytoskeleton, PHDG1A) diluted 1:140 in Antibody Diluent Reagent Solution (Invitrogen, 003218) for 30 min in the dark at room temperature

Descomps, and P

The time-dependent increase in iNOS protein level peaks at around 10 h after erastin exposure

Animal Model:FVB (Friend virus B-type) mice (Mrp4 / and WT, 6 weeks old, exposed to chronic hypoxia (10% O 2 ) in a ventilated chamber for 28 days) [2] Dosage:0, 5, and 25 mg/kg Administration:Orally, daily, for 2 more weeks, maintain in hypoxic conditions Result:Showed reversal of hypoxic pulmonary hypertension (PH), and mice were protected from hypoxic PH

The studies in which human prostate tumor samples were collected were approved by the ethical committees of the Affiliated Hospital of Qingdao University