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Additionally, based on density functional theory, we developed a computational model to predict the K d values and the trend of reaction kinetics of Michael addition reactions 30

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Bacterial cultures, or an equivalent volume of 10% (v/v) FBS-HI in Brucella broth (mock infection control), were diluted in co-culture medium (DMEM or RPMI 1640 supplemented with 10% (v/v) Brucella broth and 5% (v/v) FBS-HI) to achieve an MOI of 35 or 50