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To generate recombinant adenovirus packaging plasmids, shuttle vectors were linearized by Pme I and transformed into AdEasier competent cells

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doi: 10.1016/S0079-6123(04)48027-3

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This makes PT-141 one of the few peptides on this list with actual FDA approval in a specific context, though research-grade PT-141 is a separate product from the pharmaceutical version

We summarize potential use by injury mechanism, therapeutic timing, route of administration, monitoring strategy, safety considerations, and evidence level