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Notably, FDX1 plays a critical role in cuproptosis: when excess copper ions (particularly in the presence of copper ionophores) enter mitochondria, FDX1 reduces Cu 2+ to the more reactive Cu +

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For external calibration, we used a sodium formate solution (10 mM NaOH solution in 50/50 v/v isopropanol/water containing 0.2% formic acid) directly injected through a six-port valve at the beginning of each chromatographic run

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