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After that, the mixture was transferred into the column and pre-washed with wash buffer (PBS, contained 0, 10, or 30 mmol/L imidazole, pH = 7.6), followed by elution buffer (PBS, contained 300 mmol/L imidazole, pH = 7.6)

Notes: The MPN method as described in Standard Methods for the Examination of Water and Wastewater

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Das P, Ganguly S, Margel S, Gedanken A (2021) Immobilization of heteroatom-doped carbon dots onto nonpolar plastics for antifogging, antioxidant, and food monitoring applications

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